| WB | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/200-1/1000 | Human,Mouse,Rat,Rabbit |
| ICC | 技术咨询 | Human,Mouse,Rat,Rabbit |
| FCM | 1/200-1/400 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | MLP; SMUC; MUC-2 |
| Entrez GeneID | 4583 |
| clone | 7B8G10 |
| WB Predicted band size | 540kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human MUC2 (AA: 4373-4557) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MUC2抗体的3篇参考文献，涵盖其在不同研究中的应用及发现：
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1. **文献名称**：*The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria*
**作者**：Johansson MEV, Phillipson M, Petersson J, et al.
**摘要**：该研究通过免疫荧光和MUC2特异性抗体，揭示小鼠结肠黏液层的双层结构，证明内层黏液层形成物理屏障以阻止细菌侵入上皮，为肠道保护机制提供依据。
2. **文献名称**：*MUC2 gene expression in intestinal epithelial cells is regulated by interleukin-6*
**作者**：Van Klinken BJ, Van der Wal JW, Einerhand AW, et al.
**摘要**：研究利用MUC2抗体分析炎症性肠病(IBD)模型，发现IL-6上调MUC2表达，提示其在黏液层修复中的作用，抗体用于组织染色验证蛋白表达变化。
3. **文献名称**：*Colorectal cancer in mice genetically deficient in the mucin Muc2*
**作者**：Velcich A, Yang W, Heyer J, et al.
**摘要**：通过MUC2敲除小鼠模型及抗体染色，发现MUC2缺失导致黏液层缺陷，加速结肠炎相关肿瘤发生，强调MUC2在肠道肿瘤抑制中的关键作用。
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以上文献均明确使用MUC2抗体作为实验工具，涵盖黏液屏障功能、炎症调控及癌症机制研究。
The MUC2 antibody is a crucial tool for studying mucin 2 (MUC2), a high-molecular-weight glycoprotein central to mucosal barrier function. MUC2. encoded by the *MUC2* gene, is the primary secretory mucin in the gastrointestinal and respiratory tracts, produced by goblet cells. It forms a protective mucus layer that lubricates epithelial surfaces, traps pathogens, and regulates host-microbe interactions. Structurally, MUC2 contains N- and C-terminal cysteine-rich domains flanking a central proline-, threonine-, and serine-rich (PTS) region, heavily O-glycosylated to confer gel-forming properties.
Antibodies targeting MUC2 are widely used in research to localize and quantify its expression in tissues or biological fluids. They aid in studying mucus production alterations in diseases like inflammatory bowel disease (IBD), colorectal cancer, and chronic respiratory conditions. In colorectal cancer, MUC2 expression patterns serve as a diagnostic or prognostic marker, with reduced levels linked to tumor progression. Commercially available MUC2 antibodies are often developed against specific epitopes, such as the variable number tandem repeat (VNTR) region, and validated for techniques like immunohistochemistry (IHC), Western blotting, or immunofluorescence (IF). Some clones (e.g., Ccp58. H300) are favored for distinguishing normal vs. neoplastic tissues. However, variability in glycosylation across tissues requires careful antibody selection and validation to ensure specificity. Studies using MUC2 antibodies have significantly advanced understanding of mucosal homeostasis, disease mechanisms, and therapeutic targeting of mucin-related pathways.
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