| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Choriogonadotropin subunit beta variant 2, CGB2 |
| Entrez GeneID | 114336 |
| WB Predicted band size | 17.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CGB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CGB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACTH抗体的3篇参考文献(示例为模拟内容，实际文献需通过学术数据库检索)：
1. **文献名称**：*Development of a Highly Specific Monoclonal Antibody for ACTH Immunoassay*
**作者**：Smith A, et al.
**摘要**：研究团队开发了一种新型单克隆ACTH抗体，通过ELISA和Western blot验证其高特异性，显著降低了与类似肽段的交叉反应，适用于血浆ACTH的高灵敏度检测。
2. **文献名称**：*ACTH Antibody Localization in Pituitary Adenomas: A Comparative Study*
**作者**：Brown C, et al.
**摘要**：利用免疫组化技术，比较了两种ACTH抗体在垂体瘤样本中的染色效果，发现抗体克隆号AB-12在鉴别ACTH分泌型肿瘤中具有更高的组织特异性。
3. **文献名称**：*Validation of a Rapid ACTH Chemiluminescent Assay for Clinical Diagnostics*
**作者**：Lee D, et al.
**摘要**：该研究验证了一种基于ACTH抗体的化学发光法，可在20分钟内完成血浆ACTH定量，适用于急诊诊断库欣综合征和艾迪生病，与传统RIA法相关性达98%。
4. **文献名称**：*Cross-Reactivity of Polyclonal ACTH Antibodies: Implications for Research*
**作者**：Johnson R, et al.
**摘要**：分析了多克隆ACTH抗体与α-MSH等肽段的交叉反应性，提出通过表位筛选优化抗体设计，以减少实验假阳性结果。
(注：以上为模拟生成的参考文献，实际引用请查询PubMed、Web of Science等数据库获取真实文献。)
ACTH (adrenocorticotropic hormone) antibodies are immunochemical tools specifically designed to detect and quantify ACTH, a peptide hormone produced by the anterior pituitary gland. ACTH plays a central role in regulating cortisol secretion from the adrenal cortex via the hypothalamic-pituitary-adrenal (HPA) axis. Its measurement is critical in diagnosing disorders like Cushing's syndrome, Addison's disease, and pituitary tumors.
ACTH antibodies are typically generated in animals (e.g., rabbits, mice) using synthetic ACTH fragments or purified native ACTH as immunogens. These antibodies exhibit high specificity, distinguishing ACTH from related peptides like melanocyte-stimulating hormones (MSH) due to shared sequences in the pro-opiomelanocortin (POMC) precursor. Monoclonal antibodies offer consistent binding to defined epitopes, while polyclonal antibodies may detect multiple epitopes, enhancing sensitivity in certain assays.
In clinical and research settings, ACTH antibodies are utilized in immunoassays (e.g., ELISA, RIA, immunohistochemistry) to assess hormone levels in serum, plasma, or tissue samples. They also aid in studying HPA axis dysregulation, stress responses, and endocrine feedback mechanisms. Recent advancements focus on improving antibody affinity, cross-reactivity profiles, and compatibility with automated diagnostic platforms to enhance accuracy and throughput. Validation against standardized ACTH preparations ensures reliability across applications.
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