| WB | 1/500 - 1/2000 | Human, Monkey |
| IF | 咨询技术 | Human, Monkey |
| IHC | 咨询技术 | Human, Monkey |
| ICC | 技术咨询 | Human, Monkey |
| FCM | 1/200-1/400 | Human, Monkey |
| Elisa | 1/10000 | Human, Monkey |
| Aliases | GUD; AWT1; WAGR; WT33; NPHS4; WIT-2 |
| Entrez GeneID | 7490 |
| clone | 7F9G7H2 |
| WB Predicted band size | 49.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Monkey |
| Immunogen | Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于WT1抗体的3篇参考文献示例(注：以下信息为模拟概括，实际文献请通过学术数据库核实)：
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1. **《WT1-specific antibody response in leukemia patients after immunotherapy》**
- **作者**: T. Oka, Y. Oji, H. Sugiyama
- **摘要**: 研究探讨了白血病患者接受WT1肽疫苗治疗后产生的WT1特异性抗体反应，发现抗体水平与疾病缓解率相关，提示WT1抗体可能作为免疫治疗效果的生物标志物。
2. **《WT1 protein as a target for antibody-based cancer immunotherapy》**
- **作者**: Y. Oji, M. Nakano, H. Inoue
- **摘要**: 综述了WT1蛋白在多种实体瘤(如卵巢癌、乳腺癌)中的过表达现象，并讨论了针对WT1的单克隆抗体在靶向治疗和诊断中的应用潜力。
3. **《Clinical significance of serum WT1 antibody levels in acute myeloid leukemia》**
- **作者**: H. Inoue, K. Sugiyama, A. Tsuboi
- **摘要**: 通过ELISA检测急性髓系白血病(AML)患者血清中的WT1抗体水平，发现高抗体滴度与较低的复发风险相关，提示其预后评估价值。
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**注意事项**：
- 上述文献标题和作者为示例性内容，实际文献可能存在差异。
- 建议通过PubMed、Google Scholar等平台检索关键词“WT1 antibody”、“WT1 immunotherapy”获取最新研究。
The Wilms' tumor 1 (WT1) antibody is a diagnostic tool targeting the WT1 protein, encoded by the *WT1* gene located on chromosome 11p13. Initially identified for its role in Wilms' tumor, a pediatric kidney cancer, the WT1 gene functions as a transcription factor critical for embryonic development, particularly in the urogenital system and kidneys. While WT1 acts as a tumor suppressor in certain contexts, it exhibits oncogenic properties in others, contributing to leukemias, mesothelioma, and solid tumors.
WT1 protein overexpression is observed in various malignancies, making it a valuable biomarker. The WT1 antibody is widely used in immunohistochemistry (IHC) to detect WT1 expression in tissues, aiding in diagnosing cancers like ovarian serous carcinoma and acute leukemia. In hematopathology, it helps distinguish myeloid neoplasms from lymphoid malignancies.
Beyond diagnostics, WT1 has therapeutic relevance. WT1-derived peptides are explored in cancer vaccines, and WT1-specific antibodies are studied for targeted therapies, including CAR-T cell approaches. However, interpretation requires caution, as WT1 is expressed in normal tissues (e.g., glomerular podocytes) and some non-neoplastic conditions. Ongoing research aims to refine its clinical utility, balancing its dual roles in tumorigenesis and normal physiology.
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