| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ACTSA;α-Smooth Muscle Actin;Alpha-actin-2;Alpha actin 2 |
| Entrez GeneID | 59 |
| clone | 4G5D2 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human ACTA2 (AA: 2-124) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"ACTA2 mutations promote vascular smooth muscle cell dysfunction via increased oxidative stress"**
*Authors: Guo et al. (2020)*
**摘要**: 研究通过ACTA2抗体检测突变型α-SMA在血管平滑肌中的表达，揭示其通过ROS通路导致血管功能异常，为动脉瘤机制提供新见解。
2. **"Utility of ACTA2 antibody in identifying myofibroblast activation in liver fibrosis"**
*Authors: Li et al. (2018)*
**摘要**: 利用ACTA2抗体标记活化的肌成纤维细胞，证实其在肝纤维化模型中的特异性，支持其作为纤维化诊断的生物标志物。
3. **"ACTA2-expressing cancer-associated fibroblasts in tumor progression"**
*Authors: Chen & Wei (2021)*
**摘要**: 通过免疫组化(ACTA2抗体)分析肿瘤间质，发现高表达α-SMA的成纤维细胞与乳腺癌转移和化疗耐药性显著相关。
4. **"Smooth muscle cell-specific ACTA2 knockout reveals its role in aortic contractility"**
*Authors: Smith et al. (2019)*
**摘要**: 结合ACTA2抗体验证基因敲除模型，证明α-SMA缺失导致主动脉收缩力下降，影响血管张力调控。
(注：上述文献为示例，实际引用需根据具体研究补充完整信息。)
The α-smooth muscle actin (ACTA2) antibody is a crucial tool in biomedical research for detecting ACTA2. a protein encoded by the ACTA2 gene. ACTA2 is a cytoskeletal protein predominantly expressed in vascular smooth muscle cells (SMCs), myofibroblasts, and certain contractile cells. It plays a pivotal role in maintaining vascular tone, cell motility, and tissue contractility. In pathological conditions, ACTA2 expression is upregulated in activated myofibroblasts during fibrosis, wound healing, and cancer-associated stromal reactions, making it a marker for studying fibrotic diseases, tumor microenvironments, and vascular remodeling.
ACTA2 antibodies are widely used in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to identify SMC-rich tissues (e.g., blood vessels, hollow organs) or myofibroblast activation in conditions like atherosclerosis, cirrhosis, or solid tumors. They also aid in diagnosing smooth muscle-derived neoplasms (e.g., leiomyosarcoma) and vascular abnormalities. Additionally, ACTA2 mutations are linked to hereditary thoracic aortic aneurysms and dissections, highlighting its clinical relevance in genetic cardiovascular disorders.
As a research reagent, the antibody’s specificity and validation are critical, as cross-reactivity with other actin isoforms (e.g., β-actin) can occur. Proper controls ensure accurate interpretation in studies exploring SMC differentiation, vascular development, or therapeutic targeting of fibrotic pathways.
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