| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ACTSA;α-Smooth Muscle Actin;Alpha-actin-2;Alpha actin 2 |
| Entrez GeneID | 59 |
| clone | 5C1H11 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human ACTA2 (AA: 2-124) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ACTA2抗体的3篇参考文献及其摘要概括：
1. **"ACTA2 mutations predispose to thoracic aortic aneurysms and dissections"**
- **作者**: Guo DC, et al.
- **摘要**: 该研究通过基因测序和免疫组化分析，发现ACTA2基因突变与家族性胸主动脉瘤和夹层相关，并利用ACTA2抗体证实突变导致血管平滑肌细胞中α-SMA蛋白表达异常。
2. **"The myofibroblast: one function, multiple origins"**
- **作者**: Hinz B, et al.
- **摘要**: 本文综述了肌成纤维细胞的生物学特性，强调ACTA2抗体作为关键标记物在区分不同来源肌成纤维细胞(如伤口愈合、纤维化)中的应用，并探讨其病理意义。
3. **"ACTA2 in hepatic stellate cells promotes liver fibrosis"**
- **作者**: Kisseleva T, et al.
- **摘要**: 研究通过免疫荧光和Western blot实验，利用ACTA2抗体发现活化的肝星状细胞中α-SMA表达上调，揭示其在肝纤维化中的作用及潜在治疗靶点。
4. **"Smooth muscle actin isoforms in tumor progression"**
- **作者**: Shen T, et al.
- **摘要**: 该文献通过免疫组化分析ACTA2抗体标记的α-SMA在不同肿瘤微环境中的表达，提示其与肿瘤侵袭性和间质重塑的相关性。
以上研究均涉及ACTA2抗体的实验应用，涵盖心血管疾病、纤维化及肿瘤领域。
The ACTA2 antibody targets alpha-smooth muscle actin (α-SMA), a protein encoded by the ACTA2 gene, which is predominantly expressed in smooth muscle cells and myofibroblasts. As a key component of the cytoskeleton, α-SMA plays a critical role in cell contraction, motility, and structural integrity. It is widely used as a marker to identify smooth muscle cells in tissues such as blood vessels, the digestive tract, and the uterus. In pathological conditions, ACTA2 expression is upregulated in activated myofibroblasts during tissue repair, fibrosis, and cancer stroma, making it a valuable tool for studying fibrotic diseases, tumor microenvironments, and vascular disorders.
ACTA2 antibodies are essential in immunohistochemistry, immunofluorescence, and Western blotting to visualize α-SMA distribution and quantify its expression. Researchers utilize these antibodies to investigate diseases like atherosclerosis, liver cirrhosis, pulmonary fibrosis, and cancer progression. Notably, ACTA2 mutations are linked to hereditary aortic aneurysms and dissections (e.g., thoracic aortic disease), highlighting its clinical relevance in genetic diagnostics.
In cancer research, α-SMA-positive cancer-associated fibroblasts (CAFs) are associated with tumor aggressiveness and poor prognosis, emphasizing the antibody's utility in prognostic assessments. However, cross-reactivity with other actin isoforms requires careful validation. Overall, ACTA2 antibodies serve as pivotal tools in both basic research and clinical diagnostics, bridging molecular mechanisms with disease pathology.
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