| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MLP; SMUC; MUC-2 |
| Entrez GeneID | 4583 |
| clone | 2G4B7 |
| WB Predicted band size | 540kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MUC2 (AA: 4373-4557) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MUC2抗体的3篇参考文献及其摘要概括：
1. **"The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria"**
- **作者**: Johansson MEV et al.
- **摘要**: 该研究利用MUC2特异性抗体(通过免疫荧光和Western blot)揭示了小鼠结肠中MUC2黏液层的双层结构，证明内层黏液层通过MUC2交联形成物理屏障，有效阻隔肠道菌群侵入肠上皮。
2. **"MUC2 mucin expression in human colorectal adenocarcinomas and metastases"**
- **作者**: Ho SB et al.
- **摘要**: 通过免疫组化结合MUC2抗体，研究发现结直肠腺癌中MUC2表达显著降低，且转移灶中几乎完全缺失，提示MUC2表达缺失可能与肿瘤侵袭和转移相关。
3. **"Muc2 deficiency leads to altered expression of antimicrobial peptides in the intestinal tract"**
- **作者**: Velcich A et al.
- **摘要**: 使用MUC2基因敲除小鼠模型，通过抗体验证MUC2缺失导致肠道黏液层结构破坏，同时发现抗菌肽表达异常，表明MUC2对维持肠道菌群平衡具有关键作用。
4. **"Mucin gene expression in human intestinal mucinopathies"**
- **作者**: Tytgat KM et al.
- **摘要**: 研究利用MUC2抗体分析炎症性肠病(如溃疡性结肠炎)患者的结肠活检样本，发现MUC2表达模式异常与黏液层功能受损直接相关，为疾病机制提供了分子层面的证据。
这些文献展示了MUC2抗体在黏液屏障功能、肿瘤生物学及肠道疾病研究中的关键应用。
The MUC2 antibody targets mucin 2 (MUC2), a large, secreted glycoprotein critical for forming the protective mucus layer in epithelial tissues, particularly the gastrointestinal and respiratory tracts. MUC2 is primarily produced by goblet cells and contributes to mucosal defense by trapping pathogens, lubricating surfaces, and regulating gut microbiota interactions. Its structural complexity, characterized by tandem repeats rich in proline, threonine, and serine (PTS domains), allows extensive O-glycosylation, which is essential for its gel-forming properties.
Antibodies against MUC2 are widely used in research to study mucus biology, epithelial barrier function, and diseases linked to mucin dysregulation. For instance, altered MUC2 expression is implicated in colorectal cancer (both overexpression and loss), inflammatory bowel disease (IBD), and respiratory conditions. Monoclonal and polyclonal MUC2 antibodies enable detection of MUC2 in techniques like immunohistochemistry, Western blotting, and ELISA, helping map its distribution in healthy versus diseased tissues.
Commercial MUC2 antibodies are often validated for specificity against conserved regions, such as the N-terminal or tandem repeat domains. However, challenges persist due to MUC2’s size, glycosylation variability, and homology with other mucins. Despite this, these antibodies remain vital tools for understanding mucus-related pathologies and developing diagnostic or therapeutic strategies targeting mucosal health.
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