| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NEF; S100; S100-B; S100beta |
| Entrez GeneID | 6285 |
| clone | 4C7H12 |
| WB Predicted band size | 10.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human S100B (AA: 1-92) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于S100B抗体的模拟参考文献示例(仅供格式参考，具体内容需通过学术数据库验证)：
1. **"S100B as a biomarker for traumatic brain injury: diagnostic and prognostic utility"**
*作者：Jingjing Luo et al. (2021)*
摘要：探讨S100B蛋白在创伤性脑损伤(TBI)中的临床应用，分析其抗体检测方法的敏感性及与神经功能预后的相关性。
2. **"Development and validation of a high-specificity S100B antibody for immunohistochemistry"**
*作者：Kleindienst A, et al. (2013)*
摘要：描述一种新型S100B抗体的开发，验证其在人脑组织切片中的特异性，并比较其与传统检测方法的一致性。
3. **"S100B antibody-based detection in melanoma: implications for tumor progression monitoring"**
*作者：Smith RP, et al. (2019)*
摘要：研究S100B抗体在黑色素瘤患者血清和组织中的表达水平，评估其作为肿瘤进展和转移标志物的潜力。
4. **"The role of S100B in neurodegenerative diseases: insights from antibody-mediated inhibition studies"**
*作者：Schönthal AH, et al. (2020)*
摘要：通过抗体阻断实验探索S100B在阿尔茨海默病和帕金森病中的病理机制，提出其作为治疗靶点的可能性。
---
**注意**：以上文献信息为模拟示例，实际引用请通过PubMed、Web of Science或Google Scholar等平台检索真实文献，并核对作者、年份及摘要准确性。
The S100B antibody is a crucial tool in neuroscience and oncology research, targeting the S100B protein—a calcium-binding protein belonging to the S100 family. Primarily expressed in astrocytes and Schwann cells, S100B plays roles in cell cycle regulation, differentiation, and intracellular calcium homeostasis. It is implicated in neurological disorders (e.g., Alzheimer's, traumatic brain injury) and cancers (e.g., melanoma, gliomas). As a biomarker, elevated S100B levels in serum or cerebrospinal fluid correlate with blood-brain barrier disruption and astroglial damage.
S100B antibodies enable detection of this protein in tissues and fluids via techniques like immunohistochemistry, ELISA, and Western blot. Their specificity is critical for distinguishing S100B from homologous S100 proteins (e.g., S100A1). In research, these antibodies help elucidate S100B's dual roles: neuroprotective effects at low concentrations versus pro-inflammatory/apoptotic effects at high levels. Clinically, they aid in diagnosing melanoma metastasis and monitoring brain injury severity.
Despite utility, challenges remain due to S100B's presence in extracranial tissues and its complex interactions in pathologies. Antibody validation across species (human, rodent) is essential given S100B's conserved structure. Ongoing studies focus on standardizing assays and exploring therapeutic targeting of S100B pathways.
×
