| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OI1; OI2; OI3; OI4; EDSC; EDSARTH1 |
| Entrez GeneID | 1277 |
| clone | 7B1G12 |
| WB Predicted band size | 139kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COL1A1 (AA: 1219-1464) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与COL1A1抗体相关的文献示例及摘要概括：
1. **文献名称**：*"COL1A1 Expression in Fibrotic Disorders: A Histochemical Analysis Using Monoclonal Antibodies"*
**作者**：Smith J, et al.
**摘要**：研究通过抗COL1A1的单克隆抗体进行免疫组化分析，发现纤维化疾病(如肺纤维化、肝纤维化)患者的病变组织中COL1A1蛋白表达显著上调，提示其在细胞外基质重塑中的关键作用。
2. **文献名称**：*"Role of COL1A1 Mutations in Osteogenesis Imperfecta: Immunoblotting and Functional Studies"*
**作者**：Brown K, et al.
**摘要**：通过Western blot和免疫荧光技术，分析成骨不全症患者中COL1A1基因突变导致的胶原蛋白异常折叠，发现突变型COL1A1抗体检测显示胶原纤维组装缺陷，与疾病严重程度相关。
3. **文献名称**：*"COL1A1 as a Biomarker in Osteosarcoma: Immunohistochemical Correlations with Clinical Outcomes"*
**作者**：Lee S, et al.
**摘要**：利用抗COL1A1抗体对骨肉瘤组织进行染色，发现高表达COL1A1的肿瘤患者预后较差，提示其可作为肿瘤侵袭性和治疗抵抗的潜在标志物。
注：上述文献信息为示例，实际引用时需以真实发表的论文为准。建议通过PubMed或Web of Science以“COL1A1 antibody”为关键词检索最新研究。
The COL1A1 antibody targets the alpha-1 chain of type I collagen, a key fibrillar collagen encoded by the *COL1A1* gene. Type I collagen, the most abundant structural protein in vertebrates, forms essential extracellular matrix (ECM) components in connective tissues such as skin, bone, tendons, and organs. It provides tensile strength and supports tissue integrity. COL1A1 antibodies are widely used in research to detect and quantify type I collagen expression, aiding studies on ECM remodeling, fibrosis, skeletal disorders, and wound healing.
These antibodies are critical tools in immunohistochemistry (IHC), Western blotting, and immunofluorescence to localize collagen deposition in tissues. They help investigate pathologies like osteogenesis imperfecta (brittle bone disease), scleroderma, liver fibrosis, and tumor stroma dynamics, where collagen I overexpression or mutations are implicated. Monoclonal and polyclonal COL1A1 antibodies are available, often raised in hosts like rabbits or mice, with specificity validated via knockout controls or siRNA silencing.
Challenges include potential cross-reactivity with homologous collagen chains (e.g., COL1A2) or degraded fragments. Researchers must optimize antigen retrieval methods, particularly in formalin-fixed tissues, where collagen’s triple-helical structure may mask epitopes. Commercial COL1A1 antibodies are widely utilized, but batch variability necessitates validation using recombinant proteins or positive controls. Overall, COL1A1 antibodies remain indispensable for exploring collagen biology and related therapeutic strategies.
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