| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP |
| Entrez GeneID | 57126 |
| clone | 2F2C1 |
| WB Predicted band size | 46.4kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于CD177抗体的参考文献及其简要内容概括：
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1. **文献名称**: *CD177: A member of the Ly-6 gene family is a novel biomarker for neutrophil activation in systemic autoimmune diseases*
**作者**: Stroncek DF et al.
**摘要**: 该研究揭示CD177作为中性粒细胞特异性表面抗原，其抗体可用于检测中性粒细胞在系统性红斑狼疮(SLE)和抗中性粒细胞胞浆抗体(ANCA)相关血管炎中的激活状态，为自身免疫疾病诊断提供新标志物。
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2. **文献名称**: *CD177-mediated nanoparticle targeting of human neutrophils for enhanced drug delivery in inflammation*
**作者**: Chu D et al.
**摘要**: 文章开发了一种靶向CD177的抗体偶联纳米颗粒，证实其能特异性结合炎症部位的中性粒细胞，增强药物递送效率，为炎症性疾病治疗提供新策略。
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3. **文献名称**: *Anti-CD177 monoclonal antibodies inhibit the progression of acute myeloid leukemia by targeting leukemia stem cells*
**作者**: Li Y et al.
**摘要**: 研究报道抗CD177单克隆抗体通过阻断白血病干细胞(LSCs)表面CD177介导的生存信号通路，显著抑制急性髓系白血病(AML)模型中的肿瘤生长，提示其作为靶向治疗的潜力。
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4. **文献名称**: *CD177 modulates neutrophil transmigration by regulating β2 integrin activation*
**作者**: Bettina J et al.
**摘要**: 该研究阐明CD177通过调控β2整合素的活化影响中性粒细胞跨内皮迁移过程，抗CD177抗体可抑制这一过程，为干预中性粒细胞介导的炎症反应提供理论依据。
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以上文献涵盖CD177抗体在疾病诊断、靶向治疗及分子机制中的研究应用。
CD177. also known as human neutrophil antigen-2a (HNA-2a), is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein predominantly expressed on neutrophils. First identified in the 1990s, it plays a role in neutrophil migration to inflammatory sites, potentially mediating interactions with endothelial cells. CD177 expression varies among individuals, with approximately 3-5% of the population lacking it (HNA-2a-negative phenotype), a trait linked to genetic polymorphisms.
CD177 binds proteinase 3 (PR3), a serine protease involved in neutrophil extracellular trap (NET) formation and inflammation. This interaction localizes PR3 to the neutrophil membrane, making CD177 a key autoantigen in ANCA-associated vasculitis (AAV), where anti-PR3 antibodies contribute to vascular damage. CD177-specific antibodies are thus critical in studying AAV pathogenesis and diagnostics.
Clinically, anti-CD177 antibodies are used to detect HNA-2a antigens in blood typing, particularly in cases of neonatal alloimmune neutropenia (NAN) or transfusion-related acute lung injury (TRALI). They also serve as research tools to explore neutrophil heterogeneity, as CD177+ and CD177− subsets exhibit functional differences in inflammation and infection. Recent studies suggest CD177 may influence tumor microenvironments, with its expression observed in certain cancers, though its role remains unclear.
Overall, CD177 antibodies are vital for understanding neutrophil biology, immune disorders, and developing targeted diagnostics or therapies.
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