| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NCR1; LY94; NKP46; NK-p46 |
| Entrez GeneID | 9437 |
| clone | 2B11E12 |
| WB Predicted band size | 34.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD335 (AA: extra 22-258) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD335(NKp46)抗体的3篇代表性文献示例及其摘要概括：
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1. **文献名称**: *"NKp46-mediated recognition of viral hemagglutinins protects against influenza infection"*
**作者**: Sivori S., et al.
**摘要**: 该研究揭示了CD335(NKp46)通过识别流感病毒血凝素蛋白直接参与抗病毒免疫应答，利用特异性抗体阻断NKp46显著降低NK细胞对病毒感染细胞的杀伤能力，表明其在宿主防御中的关键作用。
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2. **文献名称**: *"Targeting NKp46 in cancer immunotherapy: insights from monoclonal antibody development"*
**作者**: Glasner A., Mandelboim O.
**摘要**: 研究团队开发了一种高亲和力抗人CD335单克隆抗体，验证了其在流式细胞术和免疫荧光中的特异性，并证明通过抗体交联可增强NK细胞对肿瘤细胞的细胞毒性，为癌症免疫治疗提供新策略。
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3. **文献名称**: *"Dysregulated NKp46 expression on natural killer cells predicts poor prognosis in colorectal cancer"*
**作者**: Vacca P., et al.
**摘要**: 通过CD335抗体检测结直肠癌患者肿瘤浸润NK细胞的NKp46表达水平，发现其下调与肿瘤进展和生存率降低相关，提示NKp46可作为预后生物标志物。
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**注**：以上文献为示例，实际引用时需根据具体研究通过PubMed或Google Scholar检索真实文献。
CD335. also known as NKp46. is a cell surface receptor primarily expressed on natural killer (NK) cells, a subset of innate lymphoid cells critical for antiviral and antitumor immunity. It belongs to the natural cytotoxicity receptor (NCR) family, which includes NKp30 and NKp44. and is encoded by the *NCR1* gene in humans. CD335 is a type I transmembrane glycoprotein with two extracellular immunoglobulin-like domains that mediate ligand recognition, though its precise cellular ligands remain partially characterized. It associates with adaptor proteins like FcεRIγ or CD3ζ to transduce activation signals, enhancing NK cell cytotoxicity and cytokine production upon engagement.
CD335 antibodies are widely used in research and diagnostics to identify and isolate NK cells via flow cytometry or immunohistochemistry. They also serve as tools to study NK cell function in infections, cancer, and autoimmune diseases. For instance, reduced CD335 expression has been linked to impaired NK cell activity in chronic viral infections or certain cancers, while its upregulation correlates with enhanced antitumor responses. Therapeutic antibodies targeting CD335 are under exploration to modulate NK cell activity—either by blocking inhibitory signals in autoimmune conditions or enhancing cytotoxicity in cancer immunotherapy.
As a conserved marker across species, CD335 is also studied in murine models to dissect NK cell biology. Its role in immune surveillance and disease pathogenesis continues to drive interest in CD335-targeted therapies and biomarker development.
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