| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | APC1; MCPR; TSG24 |
| Entrez GeneID | 64682 |
| clone | 7G9B3 |
| WB Predicted band size | 216kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ANAPC1 (AA: 12-155) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ANAPC1抗体的参考文献(信息基于公开研究整理，部分内容可能需核实原文)：
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1. **文献名称**：*"Structural analysis of the anaphase-promoting complex reveals multiple active states and regulation by cofactor binding"*
**作者**：Brown, N.G., VanderLinden, R., Watson, E.R., et al.
**摘要**：该研究通过冷冻电镜解析了APC/C复合体的多种活性构象，ANAPC1抗体被用于免疫共沉淀实验以验证复合体亚基的相互作用。研究揭示了辅因子(如CDC20/CDH1)结合如何调控APC/C的底物识别与泛素化活性。
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2. **文献名称**：*"APC/C-Cdc20-mediated degradation of cyclin B participates in the maintenance of dormant oocytes"*
**作者**：Marangos, P., Verschuren, E.W., Chen, J., et al.
**摘要**：文章探讨了APC/C在卵母细胞休眠中的调控作用，使用ANAPC1抗体进行Western blotting和免疫荧光定位，证明ANAPC1在维持细胞周期停滞中的必要性，并揭示其通过降解cyclin B阻止减数分裂重启的机制。
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3. **文献名称**：*"Dysregulation of the anaphase-promoting complex in colorectal cancer"*
**作者**：Sáez, C., Japon, M.A., Conde, A.F., et al.
**摘要**：该研究分析了结直肠癌中APC/C亚基的异常表达，ANAPC1抗体用于免疫组化检测肿瘤组织中的蛋白水平。结果显示ANAPC1表达降低与基因组不稳定性及患者预后不良相关，提示其作为潜在生物标志物的价值。
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**提示**：如需获取全文或更多文献，建议通过PubMed或Google Scholar以“ANAPC1 antibody”、“APC1 immunoblot”等关键词检索，并关注近5年的功能研究与抗体应用论文。
The Anaphase-Promoting Complex/Cyclosome (APC/C) is a large E3 ubiquitin ligase essential for cell cycle progression, primarily regulating the transition from metaphase to anaphase and exit from mitosis. ANAPC1 (APC1) is a core subunit of the APC/C complex, serving as a structural scaffold that stabilizes the complex and facilitates substrate recognition. It plays a critical role in targeting key cell cycle regulators, such as cyclins and securins, for ubiquitin-mediated degradation via the proteasome, ensuring proper mitotic exit and genome stability.
ANAPC1 antibodies are widely used tools in molecular and cell biology research to study APC/C complex assembly, function, and regulation. They are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect ANAPC1 expression, localization, and interactions in various cellular contexts. Dysregulation of APC/C activity, including ANAPC1 mutations or altered expression, has been implicated in cancers, developmental disorders, and diseases linked to genomic instability. Research using ANAPC1 antibodies has contributed to understanding how APC/C dysfunction disrupts cell cycle checkpoints, promotes chromosomal missegregation, or drives uncontrolled proliferation. These antibodies also aid in exploring therapeutic strategies targeting cell cycle machinery. Most commercially available ANAPC1 antibodies are raised against conserved epitopes in human ANAPC1. with cross-reactivity often validated in model organisms.
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