| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BAK; CDN1; BCL2L7; BAK-LIKE |
| Entrez GeneID | 578 |
| clone | 2H9H7 |
| WB Predicted band size | 23.4kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BAK1 (AA: 29-187) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于BAK1抗体的3篇文献示例(内容为模拟虚构，仅供参考)：
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1. **文献名称**: *Conformational changes in BAK1 during apoptosis revealed by a specific monoclonal antibody*
**作者**: Zhang L, et al.
**摘要**: 本研究开发了一种针对BAK1构象表位的单克隆抗体，用于检测其在凋亡过程中的激活状态。实验表明，该抗体能特异性识别BAK1的寡聚化构象，证实了BAK1在线粒体外膜穿孔中的关键作用。
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2. **文献名称**: *BAK1 antibody-based detection of protein interactions in plant immune signaling*
**作者**: Wang Y, et al.
**摘要**: 通过BAK1多克隆抗体进行免疫共沉淀实验，揭示了BAK1与植物免疫受体FLS2的相互作用机制，为植物先天免疫信号通路研究提供了工具支持。
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3. **文献名称**: *Differential roles of BAK1 and BAX in chemotherapy-induced apoptosis using isoform-specific antibodies*
**作者**: Miller R, et al.
**摘要**: 利用亚型特异性抗体区分BAK1和BAX的功能，发现BAK1在结肠癌细胞凋亡中早于BAX激活，其抗体阻断实验进一步验证了二者协同促凋亡的机制。
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注：以上文献信息为示例，实际引用需根据真实发表的论文调整。建议通过PubMed或Google Scholar搜索关键词“BAK1 antibody”或“BAK1 antibody application”获取准确文献。
BAK1 (BRI1-Associated Receptor Kinase 1), also known as SERK3 (Somatic Embryogenesis Receptor Kinase 3), is a leucine-rich repeat receptor-like kinase (LRR-RLK) that functions as a critical co-receptor in plant signaling pathways. Initially identified for its role in brassinosteroid (BR) signaling through interaction with the BR receptor BRI1. BAK1 is now recognized as a multifunctional regulator in plant innate immunity and development. It forms ligand-induced complexes with pattern recognition receptors (PRRs) like FLS2 and EFR, mediating responses to pathogen-associated molecular patterns (PAMPs) such as flagellin and EF-Tu, thereby activating PAMP-triggered immunity (PTI).
BAK1 antibodies are essential tools for studying its protein expression, localization, and interaction dynamics. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate BAK1's involvement in immune signaling cascades, cell death regulation, and stress responses. These antibodies are typically raised against specific epitopes of BAK1. often in rabbits or mice, and require rigorous validation for specificity due to high homology with other SERK family members. Researchers rely on BAK1 antibodies to explore its phosphorylation status, subcellular distribution, and tissue-specific expression under various biotic/abiotic stresses. Their applications extend to genetic studies, including mutant phenotyping and transgenic plant analysis, providing insights into BAK1's dual roles in growth-defense trade-offs. Proper controls, such as using bak1 mutant lines, are critical to avoid cross-reactivity artifacts.
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