| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BCLW; BCL-W; PPP1R51; BCL2-L-2 |
| Entrez GeneID | 599 |
| clone | 4G12D11 |
| WB Predicted band size | 20.7kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BCL2L2 (AA: 6-118) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于BCL2L2抗体的3篇参考文献(摘要内容已简化概括)：
---
1. **文献名称**：*BCL2L2 (Bcl-w) promotes survival of breast cancer cells*
**作者**：Lee et al.
**摘要**：研究利用特异性BCL2L2抗体检测乳腺癌组织中Bcl-w蛋白表达，发现其高表达与细胞抗凋亡能力增强相关，提示其在乳腺癌进展中的作用。
---
2. **文献名称**：*Development of a monoclonal antibody specific for BCL2L2 and its application in immunohistochemistry*
**作者**：Smith et al.
**摘要**：报道一种新型抗BCL2L2单克隆抗体的开发，验证其在免疫组化(IHC)中的特异性，并应用于结肠癌组织样本分析，证实BCL2L2在肿瘤细胞中的过表达。
---
3. **文献名称**：*The role of BCL2L2 in regulating mitochondrial apoptosis pathways*
**作者**：Chen et al.
**摘要**：通过Western blot(使用商业化BCL2L2抗体)和基因敲除技术，揭示BCL2L2通过抑制线粒体凋亡通路保护神经元细胞，为神经退行性疾病研究提供依据。
---
注：以上文献信息为模拟示例，实际引用需根据具体论文调整。建议通过PubMed或Web of Science搜索关键词“BCL2L2 antibody”或“Bcl-w antibody”获取最新文献。
The BCL2L2 antibody targets the BCL2-like 2 protein (BCL2L2), also known as BCL-W, a member of the BCL-2 family of apoptosis regulators. BCL-W is an anti-apoptotic protein that promotes cell survival by binding and neutralizing pro-apoptotic family members like BAX and BAK, thereby preventing mitochondrial outer membrane permeabilization and caspase activation. It is broadly expressed in tissues, with elevated levels observed in certain cancers, including colorectal, breast, and gastric cancers, where it contributes to tumor progression and chemoresistance.
Antibodies against BCL2L2 are critical tools for studying its expression, localization, and interactions in both physiological and pathological contexts. They are widely used in techniques such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to assess BCL-W levels in cell lines, tissues, or clinical samples. Such studies help elucidate its role in apoptosis regulation, cancer biology, and potential as a therapeutic target. For instance, BCL2L2 antibodies aid in evaluating the efficacy of BH3-mimetic drugs designed to disrupt anti-apoptotic BCL-2 protein functions.
Research using BCL2L2 antibodies has also explored its involvement in non-cancerous conditions, such as neurodegenerative diseases and autoimmune disorders, where dysregulated apoptosis pathways may contribute to pathogenesis. These antibodies are essential for advancing both basic research and preclinical drug development targeting BCL-W-mediated survival mechanisms.
×
