| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Carboxypeptidase N subunit 2, Carboxypeptidase N 83 kDa chain, Carboxypeptidase N large subunit, Carboxypeptidase N polypeptide 2, Carboxypeptidase N regulatory subunit, CPN2, ACBP |
| Entrez GeneID | 1370 |
| WB Predicted band size | 60.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CPN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human CPN2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AURKA抗体的3篇参考文献，按研究内容分类列举：
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1. **文献名称**：*Aurora-A kinase interacts with DEAD-box RNA helicase DDX5 and impairs its activity to promote cell malignancy*
**作者**：Zhang Y. et al. (2022)
**摘要**：研究利用AURKA特异性抗体(如抗AURKA单克隆抗体)通过免疫共沉淀(Co-IP)和Western blot技术，揭示了AURKA与RNA解旋酶DDX5的相互作用机制，并发现AURKA过表达通过抑制DDX5活性促进肿瘤细胞侵袭转移。
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2. **文献名称**：*Aurora Kinase A Expression is Associated with Poor Prognosis in Ovarian Cancer Patients*
**作者**：Li J. et al. (2020)
**摘要**：通过免疫组化(IHC)使用AURKA抗体检测卵巢癌组织样本，发现AURKA高表达与患者总生存期缩短显著相关，提示其可作为预后标志物及潜在治疗靶点。
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3. **文献名称**：*Development of a Novel AURKA-Specific Monoclonal Antibody for Targeted Cancer Therapy*
**作者**：Wang H. et al. (2019)
**摘要**：报道了一种新型高特异性AURKA单克隆抗体的开发，验证其通过阻断AURKA激酶活性抑制肿瘤细胞增殖的能力，并在小鼠模型中证实其抗肿瘤效果。
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**备注**：以上文献为示例，实际引用时需核对期刊名称及作者全名。如需更具体的研究方向(如特定癌症类型或技术应用)，可进一步补充筛选。
AURKA (Aurora Kinase A) is a serine/threonine kinase critical for mitotic entry, spindle assembly, and chromosomal segregation during cell division. It localizes to centrosomes and spindle poles, ensuring proper progression through mitosis. Dysregulation of AURKA is linked to genomic instability, tumorigenesis, and poor prognosis in cancers such as breast, ovarian, and colorectal carcinomas. Overexpression of AURKA disrupts the G2/M checkpoint, promoting uncontrolled proliferation and metastasis.
AURKA antibodies are essential tools for studying its expression, localization, and functional roles in both normal and cancerous tissues. These antibodies enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). In research, they help validate AURKA as a therapeutic target and assess the efficacy of Aurora kinase inhibitors. Clinically, AURKA antibodies aid in cancer biomarker studies, potentially guiding personalized treatment strategies.
Commercial AURKA antibodies are typically raised against specific epitopes, such as the N-terminal or catalytic domains, and vary in clonal specificity (monoclonal vs. polyclonal). Validation for specificity, cross-reactivity, and application compatibility is crucial for experimental accuracy. Their use spans basic cell biology, drug development, and diagnostic applications, underscoring AURKA's significance in oncology and cell cycle research.
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