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Mouse Monoclonal ASF1B Antibody

  • 中文名: ASF1B抗体
  • 别    名: CIA-II
货号: IPD31191
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500 - 1/2000 Human,Mouse,Monkey
IF 咨询技术 Human,Mouse,Monkey
IHC 咨询技术 Human,Mouse,Monkey
ICC 技术咨询 Human,Mouse,Monkey
FCM 1/200 - 1/400 Human,Mouse,Monkey
Elisa 1/10000 Human,Mouse,Monkey

产品详情

AliasesCIA-II
Entrez GeneID55723
clone6G7G4
WB Predicted band size22.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Monkey
ImmunogenPurified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是关于ASF1B抗体的3篇参考文献的简要概括:

1. **文献名称**:*ASF1B promotes oncogenesis in lung adenocarcinoma via regulation of cell cycle and DNA repair*

**作者**:Zhang Y, et al.

**摘要**:该研究利用ASF1B特异性抗体进行免疫组化分析,发现ASF1B在肺腺癌中高表达,并通过调控细胞周期和DNA修复通路促进肿瘤进展。

2. **文献名称**:*Development and validation of a novel anti-ASF1B monoclonal antibody for chromatin assembly studies*

**作者**:Thompson R, et al.

**摘要**:研究团队开发并验证了一种高特异性ASF1B单克隆抗体,通过Western blot和免疫荧光证实其在染色质组装研究中的应用价值,为机制研究提供工具支持。

3. **文献名称**:*ASF1B expression correlates with poor prognosis in triple-negative breast cancer*

**作者**:Li H, et al.

**摘要**:通过ASF1B抗体进行组织微阵列分析,研究发现ASF1B在三阴性乳腺癌中高表达,与患者生存率负相关,提示其作为潜在预后标志物的可能性。

注:以上文献信息为模拟示例,实际引用需以具体数据库(如PubMed)检索结果为准。

背景信息

The ASF1B (Anti-silencing Function 1B) antibody targets a histone chaperone protein critical for chromatin assembly and disassembly during DNA replication and repair. ASF1B, a paralog of ASF1A, binds histone H3-H4 dimers, facilitating their deposition onto DNA or transfer to other chaperones. It plays a key role in cell cycle progression, particularly in S phase and mitotic chromosome segregation. Unlike ASF1A, which is ubiquitously expressed, ASF1B is strongly upregulated in proliferating cells and overexpressed in various cancers, linking it to tumorigenesis and poor prognosis. Researchers use ASF1B antibodies to study its nuclear localization, interactions with replication machinery (e.g., MCM2-7 complex), and roles in maintaining genomic stability. These antibodies are validated for techniques like Western blotting, immunofluorescence, and ChIP-seq. Recent studies explore ASF1B's potential as a cancer biomarker and therapeutic target, given its association with chemotherapy resistance. Specificity validation against ASF1A is essential due to high sequence homology between the isoforms.

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