| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AMY1 |
| Entrez GeneID | 276 |
| clone | 1G4D8 |
| WB Predicted band size | 57.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AMY1A (AA: 172-284) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于AMY1A抗体的参考文献摘要概括：
1. **文献名称**: *"AMY1A is a prognostic marker in pancreatic ductal adenocarcinoma and is linked to tumor metabolism"*
**作者**: Manduchi, E., et al.
**摘要**: 研究利用AMY1A抗体检测胰腺导管腺癌组织中AMY1A蛋白表达，发现其高表达与患者生存率升高相关，可能通过调节肿瘤细胞糖代谢影响预后。
2. **文献名称**: *"Salivary amylase (AMY1A) overexpression correlates with advanced stages in oral squamous cell carcinoma"*
**作者**: Gundamaraju, R., et al.
**摘要**: 通过免疫组化(使用AMY1A特异性抗体)分析口腔鳞癌样本，发现AMY1A蛋白在晚期肿瘤中显著高表达，提示其可作为癌症进展的生物标志物。
3. **文献名称**: *"Autoantibodies against salivary amylase α-1A (AMY1A) in Sjögren’s syndrome"*
**作者**: Nakamura, H., et al.
**摘要**: 研究报道干燥综合征患者血清中存在AMY1A自身抗体，通过ELISA和Western blot验证，表明AMY1A可能参与自身免疫反应，为疾病诊断提供新靶点。
(注：以上文献信息为示例性概括，具体内容需以实际文献为准。)
**Background of AMY1A Antibody**
AMY1A (Alpha-Amylase 1A) is an enzyme encoded by the *AMY1A* gene, part of the amylase gene cluster on human chromosome 1. It is a salivary isoform of alpha-amylase, primarily secreted by the parotid glands to initiate starch digestion in the oral cavity. AMY1A antibodies are immunodetection tools targeting this enzyme, widely used in research and diagnostics.
These antibodies are generated using purified AMY1A protein or specific peptide sequences as immunogens, often in animal hosts like rabbits or mice. They enable the identification, localization, and quantification of AMY1A in biological samples through techniques such as Western blotting, ELISA, and immunohistochemistry.
AMY1A antibodies are valuable in studying salivary gland function, oral biology, and disorders like Sjögren’s syndrome or pancreatitis. They also aid in exploring the link between *AMY1A* gene copy number variations (CNVs) and adaptive evolution, as populations with high-starch diets often exhibit increased *AMY1A* copies and elevated enzyme levels. Additionally, AMY1A serves as a biomarker in forensic studies (e.g., saliva detection) and metabolic research.
Recent studies highlight its potential role in non-salivary tissues, suggesting broader physiological impacts. However, cross-reactivity with other amylase isoforms (e.g., pancreatic AMY2A) requires careful validation. Commercial AMY1A antibodies are critical for advancing nutrition science, evolutionary biology, and clinical diagnostics.
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