| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1 |
| Entrez GeneID | 9370 |
| clone | 1E10D5 |
| WB Predicted band size | 26.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ADIPOQ (AA: 16-154) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ADIPOQ抗体的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Development of a High-Specificity ELISA for Human Adiponectin Using Monoclonal Antibodies"*
**作者**: Tanaka S, et al.
**摘要**: 本研究开发了一种基于单克隆ADIPOQ抗体的新型ELISA检测方法，用于定量人血清中的脂联素。抗体针对脂联素C端结构域，验证显示高灵敏度和低交叉反应性，适用于肥胖和糖尿病患者的临床样本分析。
2. **文献名称**: *"Comparative Analysis of Commercial Anti-Adiponectin Antibodies in Western Blot Applications"*
**作者**: Müller R, et al.
**摘要**: 评估了5种商用ADIPOQ抗体在不同物种组织提取物中的性能，发现部分抗体存在非特异性结合问题。研究提出了优化Western Blot实验条件的建议，以提高脂联素检测的准确性。
3. **文献名称**: *"Adiponectin Localization in Human Adipose Tissue Using Immunohistochemistry: Technical Considerations and Biological Implications"*
**作者**: Lee H, et al.
**摘要**: 通过优化ADIPOQ抗体的抗原修复和封闭步骤，实现了脂联素在脂肪组织切片中的特异性染色。研究发现脂联素表达与脂肪细胞大小呈负相关，提示其在代谢调控中的作用。
4. **文献名称**: *"Role of Adiponectin in Cardiovascular Disease: Insights from Antibody-Based Neutralization Studies"*
**作者**: Zhang Y, et al.
**摘要**: 使用ADIPOQ中和抗体在小鼠模型中阻断脂联素功能，发现其加剧动脉粥样硬化进展，证实了脂联素在心血管保护中的直接作用。
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**提示**：实际引用时建议通过PubMed或Google Scholar检索关键词“ADIPOQ antibody”、“adiponectin ELISA”或结合研究领域(如“obesity”“diabetes”)，并筛选近年高被引文献以确保权威性。
ADIPOQ, also known as adiponectin, is an adipocyte-secreted hormone encoded by the ADIPOQ gene. It plays a critical role in regulating glucose and fatty acid metabolism by enhancing insulin sensitivity, suppressing inflammation, and promoting energy homeostasis. Adiponectin exists in multiple isoforms (low-molecular-weight, medium, and high-molecular-weight oligomers) and binds to receptors AdipoR1 and AdipoR2 to activate signaling pathways like AMPK and PPAR-α. Reduced circulating adiponectin levels are associated with obesity, type 2 diabetes, cardiovascular diseases, and metabolic syndrome.
ADIPOQ antibodies are essential tools for detecting and quantifying adiponectin in research and diagnostics. These antibodies, often polyclonal or monoclonal, target specific epitopes within adiponectin's conserved domains (e.g., collagen-like or globular regions). They are widely used in immunoassays (ELISA, Western blotting) and immunohistochemistry to study adipokine expression patterns, tissue distribution, and its role in metabolic disorders. Some antibodies distinguish between total adiponectin and its oligomeric forms, aiding in functional studies. Validation for specificity and cross-reactivity is crucial, as adiponectin shares structural homology with complement factor C1q.
Research using ADIPOQ antibodies has advanced understanding of adipose tissue dysfunction, therapeutic interventions, and biomarker development for metabolic diseases.
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