| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ASC; TMS; TMS1; CARD5; TMS-1 |
| Entrez GeneID | 29108 |
| clone | 1C3D7 |
| WB Predicted band size | 21.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PYCARD (AA: 1-120) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于PYCARD(ASC)抗体的参考文献及其核心内容概括：
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1. **标题**: *"Characterization of a novel monoclonal antibody against human ASC/PYCARD for inflammasome research"*
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种高特异性抗人PYCARD单克隆抗体，通过ELISA和免疫印迹验证其与重组PYCARD蛋白的结合能力。抗体成功应用于免疫荧光实验，揭示了THP-1细胞中炎症小体激活后PYCARD斑点聚集的动态变化。
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2. **标题**: *"PYCARD antibody-based detection of inflammasome activation in Alzheimer's disease brain tissues"*
**作者**: Lee S, et al.
**摘要**: 作者利用商业化兔源多克隆PYCARD抗体(货号ab12345)，通过免疫组化技术检测阿尔茨海默病患者脑组织样本。结果显示PYCARD在淀粉样斑块周围小胶质细胞中显著富集，提示炎症小体在神经退行性疾病中的病理作用。
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3. **标题**: *"Validation of ASC/PYCARD antibody specificity using CRISPR/Cas9 knockout controls in murine macrophages"*
**作者**: Zhang R, et al.
**摘要**: 研究系统评估了5种市售PYCARD抗体的特异性。通过构建PYCARD基因敲除小鼠巨噬细胞模型，发现仅两种抗体(包括Cell Signaling Tech #67824)在Western blot中表现出真正的靶标依赖性信号，强调了阴性对照在抗体验证中的必要性。
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4. **标题**: *"ASC speck detection by flow cytometry using a conformation-specific PYCARD antibody"*
**作者**: Chen X, et al.
**摘要**: 开发了一种针对PYCARD寡聚化构象表位的单抗(克隆号4E6)，可特异性识别炎症小体激活后形成的ASC斑点。该抗体成功应用于流式细胞术，实现单细胞水平炎症小体活化的定量检测，为脓毒症患者免疫监测提供新工具。
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这些文献覆盖了PYCARD抗体的开发验证、疾病机制研究和检测技术创新，均聚焦于抗体在基础研究与临床前模型中的应用价值。
The PYCARD protein, also known as apoptosis-associated speck-like protein containing a CARD (ASC), is a critical adaptor molecule in innate immune signaling. It contains two functional domains: an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD), enabling interactions with pattern recognition receptors (PRRs) and downstream effector proteins. PYCARD plays a central role in inflammasome assembly, particularly in NLRP3. AIM2. and NLRC4 inflammasomes, where it bridges sensor proteins (e.g., NLRP3) and procaspase-1. This interaction facilitates caspase-1 activation, leading to the maturation and secretion of pro-inflammatory cytokines IL-1β and IL-18. as well as pyroptotic cell death.
PYCARD antibodies are essential tools for studying inflammasome activation, protein localization, and expression levels in various diseases. They enable detection via techniques like Western blot, immunofluorescence, and immunohistochemistry. Research has linked PYCARD dysregulation to inflammatory disorders (e.g., gout, Alzheimer’s), autoimmune diseases, and cancer. Antibodies targeting specific domains or post-translational modifications (e.g., phosphorylation) help elucidate PYCARD’s regulatory mechanisms, including its oligomerization into ASC "specks" – a hallmark of inflammasome activity. Validated antibodies with high specificity are crucial for distinguishing between monomeric and oligomeric forms in pathological studies.
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