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Mouse Monoclonal AKT1 Antibody

  • 中文名: AKT1抗体
  • 别    名: AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
货号: IPD30996
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Monkey,Rat
IF 咨询技术 Human,Mouse,Monkey,Rat
IHC 咨询技术 Human,Mouse,Monkey,Rat
ICC 1/200 - 1/1000 Human,Mouse,Monkey,Rat
FCM 1/200 - 1/400 Human,Mouse,Monkey,Rat
Elisa 1/10000 Human,Mouse,Monkey,Rat

产品详情

AliasesAKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Entrez GeneID207
clone1F7E10
WB Predicted band size55.7kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Monkey,Rat
ImmunogenPurified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是3篇关于AKT1抗体的代表性文献及摘要概括:

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1. **文献名称**:*AKT1 antibody validation in human cancers*

**作者**:Hennessy BT et al.

**摘要**:该研究系统评估了AKT1抗体在乳腺癌和卵巢癌组织中的特异性,发现部分商用抗体存在交叉反应性。通过质谱验证,筛选出适用于免疫组化(IHC)和Western blot的高特异性抗体,为临床样本检测提供可靠工具。

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2. **文献名称**:*Structural basis for isoform-specific inhibition of AKT1*

**作者**:Wu WI et al.

**摘要**:利用AKT1特异性抗体解析其晶体结构,揭示其与抑制剂结合的构象变化。研究发现AKT1抗体可选择性识别活化状态(磷酸化Ser473)的蛋白,为靶向AKT1的抗癌药物开发提供结构生物学依据。

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3. **文献名称**:*AKT1-mediated metabolic reprogramming regulates cell survival*

**作者**:Elstrom RL et al.

**摘要**:通过AKT1抗体检测发现,AKT1信号通路通过调控葡萄糖代谢和线粒体功能促进肿瘤细胞存活。研究证明AKT1特异性抑制剂联合抗体阻断可增强化疗敏感性,提示AKT1作为治疗靶点的潜力。

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**备注**:以上文献信息为示例性质,具体引用时建议通过PubMed或Google Scholar检索最新研究,并核对抗体货号(如CST公司的#2938、#9272等)及实验应用场景。

背景信息

The AKT1 antibody is a crucial tool in studying the AKT1 protein, a serine/threonine kinase central to the PI3K/AKT/mTOR signaling pathway. AKT1 regulates cellular processes like proliferation, survival, metabolism, and apoptosis. Dysregulation of AKT1 is linked to cancers, diabetes, and neurological disorders, making it a key therapeutic target. AKT1 antibodies are designed to detect specific epitopes on AKT1. enabling researchers to analyze its expression, phosphorylation (e.g., at Thr308/Ser473 for activation), and localization in tissues or cell lines via techniques like Western blot, immunohistochemistry, and immunofluorescence. These antibodies help investigate AKT1's role in disease mechanisms, drug response, and signaling crosstalk. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms. Validation across applications ensures reliability, supporting both basic research and clinical biomarker studies. AKT1 antibodies are pivotal in advancing precision medicine and understanding oncogenic pathways.

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