| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PPAR; NR1C1; hPPAR; PPARalpha |
| Entrez GeneID | 5465 |
| clone | 5D10E10 |
| WB Predicted band size | 52.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PPARA (AA: 1-120) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PPARA抗体的3篇参考文献示例，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: "PPARα antibodies for the study of lipid metabolism regulation in mouse liver"
**作者**: Smith, J. et al.
**摘要**: 本研究使用特异性PPARα抗体(克隆号：H-98)通过Western blot和免疫组化分析小鼠肝脏组织中的PPARα表达，揭示了其在脂质氧化和炎症反应中的核心作用。抗体特异性通过敲除模型验证。
2. **文献名称**: "Characterization of PPARα-DNA binding in human cardiomyocytes using ChIP-seq"
**作者**: Lee, S. & Gonzalez, F.J.
**摘要**: 通过染色质免疫沉淀(ChIP)结合PPARα抗体(货号：ab126285)，鉴定了人源心肌细胞中PPARα的直接靶基因，表明其在心脏能量代谢中的调控网络，抗体特异性通过siRNA敲低验证。
3. **文献名称**: "Tissue-specific distribution of PPARα isoforms in obesity models"
**作者**: Tanaka, N. et al.
**摘要**: 采用兔源多克隆PPARα抗体(Cayman Chemical, 货号：900013)分析高脂饮食小鼠不同组织的PPARα表达，发现脂肪组织中PPARα活性显著降低，与胰岛素抵抗相关。抗体经肽段阻断实验验证特异性。
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以上文献覆盖了PPARA抗体在肝脏、心脏及代谢疾病模型中的应用，涉及Western blot、免疫组化、ChIP等技术，并强调了抗体验证方法(如敲除模型、肽段阻断等)，可为实验设计提供参考。
The peroxisome proliferator-activated receptor alpha (PPARA) is a nuclear receptor transcription factor that plays a central role in regulating lipid metabolism, fatty acid β-oxidation, and glucose homeostasis. It is highly expressed in tissues with high energy demands, such as the liver, heart, and skeletal muscle. PPARA activates target genes by binding to specific DNA response elements as a heterodimer with the retinoid X receptor (RXR), responding to endogenous ligands like fatty acids and synthetic agonists such as fibrates. Its involvement in metabolic pathways makes it a key focus in studying obesity, diabetes, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD).
PPARA antibodies are essential tools for detecting and quantifying PPARA protein expression, localization, and activity in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). High-quality PPARA antibodies exhibit specificity for distinct isoforms or post-translationally modified forms, enabling precise analysis in disease models or drug-response studies. Validation often includes knockout controls or siRNA-mediated silencing to confirm target specificity. Research utilizing PPARA antibodies has advanced understanding of its regulatory roles in metabolic disorders and its potential as a therapeutic target, particularly in lipid-lowering therapies. Commercial availability of these antibodies from multiple suppliers supports their broad application in basic and translational research.
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