| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TIP; ESA1; PLIP; KAT5; cPLA2; HTATIP; ZC2HC5; HTATIP1 |
| Entrez GeneID | 10524 |
| clone | 2A11H7 |
| WB Predicted band size | 58.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TIP60 (AA: 18-208) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于TIP60抗体的参考文献及其摘要内容概括：
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1. **文献名称**: "Role of the TIP60 acetyltransferase in DNA repair and apoptosis"
**作者**: Ikura T, et al.
**摘要**: 该研究使用TIP60抗体进行免疫沉淀和染色质免疫共沉淀(ChIP)实验，证实TIP60通过乙酰化组蛋白H4参与DNA双链断裂修复，并调控下游凋亡信号通路。
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2. **文献名称**: "TIP60 interacts with the androgen receptor to enhance its transcriptional activity in prostate cancer"
**作者**: Hsu FN, et al.
**摘要**: 通过Western blot和免疫荧光实验(使用TIP60特异性抗体)，研究发现TIP60与雄激素受体(AR)结合，增强其转录活性，促进前列腺癌细胞增殖和侵袭。
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3. **文献名称**: "Selective inhibition of TIP60 acetyltransferase activity suppresses glioblastoma growth"
**作者**: Wang Y, et al.
**摘要**: 利用TIP60抗体检测其在胶质母细胞瘤中的表达水平，发现靶向抑制TIP60的乙酰转移酶活性可诱导肿瘤细胞周期阻滞，为治疗提供潜在策略。
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以上文献均涉及TIP60抗体的实验应用(如Western blot、ChIP等)，并涵盖其在DNA修复、癌症调控等领域的功能研究。如需具体文献来源，建议通过PubMed或期刊官网检索标题获取全文。
The TIP60 (Tat-interactive protein 60) antibody is a crucial tool for studying the function and regulation of the TIP60 protein, a member of the MYST family of histone acetyltransferases (HATs). TIP60 plays a pivotal role in chromatin remodeling, DNA repair, cell cycle regulation, and apoptosis by acetylating histones (e.g., H4 and H2A) and non-histone targets, thereby modulating gene expression and protein interactions. It is also involved in critical signaling pathways, including the DNA damage response, where it interacts with tumor suppressors like p53.
TIP60 antibodies are widely used in research to detect and quantify TIP60 expression via techniques such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). These antibodies are available in monoclonal and polyclonal forms, often raised in hosts like rabbits or mice, with specificity validated through knockout controls or competitive assays. Researchers rely on TIP60 antibodies to investigate its role in cancer (where it can act as both oncogene and tumor suppressor), epigenetic regulation, and cellular stress responses.
Due to its diverse functions, TIP60 antibody specificity is critical, as cross-reactivity with other MYST family members may occur. Proper validation ensures accurate interpretation in studies exploring TIP60's involvement in diseases or its therapeutic targeting.
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