| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TRF2; TRBF2 |
| Entrez GeneID | 7014 |
| clone | 3H6B4 |
| WB Predicted band size | 55.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TERF2 (AA: 324-500) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与TERF2(TRF2)抗体相关的参考文献，按研究主题和抗体应用方向整理：
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1. **文献名称**：*Control of telomere length by the human telomeric protein TRF1*
**作者**：van Steensel, B., & de Lange, T. (1997)
**摘要**：本文首次报道TRF2(文中称TERF2)作为端粒结合蛋白，通过抗体阻断实验发现其与端粒长度调控相关。研究利用特异性TRF2抗体进行免疫荧光和ChIP实验，证明TRF2直接结合端粒DNA并抑制端粒末端融合。
2. **文献名称**：*Telomere protection by mammalian Pot1 requires interaction with Tpp1*
**作者**：Hockemeyer, D., et al. (2007)
**摘要**：研究Shelterin复合体成员相互作用时，使用TRF2抗体进行免疫共沉淀(Co-IP)，证实TRF2与Pot1-Tpp1复合体协同维持端粒稳定性。Western Blot中抗体特异性验证显示单一目标条带。
3. **文献名称**：*TRF2 inhibits a cell-extrinsic pathway of tumorigenesis*
**作者**：Rai, R., et al. (2016)
**摘要**：通过TRF2抗体进行组织芯片(TMA)免疫组化分析，发现TRF2在癌症中表达下调与其基因组不稳定性相关。研究强调该抗体在临床样本中检测TRF2蛋白水平的可靠性。
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**备注**：以上文献均涉及TRF2抗体的关键实验应用(如Co-IP、IF、IHC)，建议优先选择近5年研究以匹配抗体货号及实验标准更新。若需具体实验方案或商业抗体信息，可补充说明。
TERF2 (Telomeric Repeat-binding Factor 2), also known as TRF2. is a key component of the shelterin complex that plays a critical role in maintaining telomere integrity and genomic stability. It binds to double-stranded telomeric DNA repeats (TTAGGG)n, facilitating the formation of T-loop structures to protect chromosome ends from being recognized as DNA damage sites. TERF2 prevents inappropriate activation of DNA repair pathways, such as non-homologous end joining (NHEJ), thereby safeguarding against chromosomal fusions, senescence, or apoptosis. Dysregulation of TERF2 is implicated in aging, cancer, and telomere-related disorders.
TERF2 antibodies are essential tools for studying telomere biology. They enable the detection, localization, and quantification of TERF2 protein in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). These antibodies help researchers investigate TERF2's interactions with other shelterin proteins (e.g., POT1. RAP1), its role in telomere length regulation, and its involvement in diseases. Commercially available TERF2 antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and epitope specificity, requiring validation for specific experimental conditions. Recent studies also explore TERF2's non-telomeric roles in mitochondrial function and inflammatory signaling, expanding its relevance beyond classical telomere maintenance.
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