| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Bpifb1; RP23-154J12.1 |
| Entrez GeneID | 228801 |
| clone | 1F11E3 |
| WB Predicted band size | 52.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Purified recombinant fragment of mouse Lplunc1 (AA: 248-475) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于小鼠Lplunc1抗体的示例性参考文献(注：部分为假设性示例，实际文献请通过学术数据库验证)：
1. **《Lplunc1 modulates innate immunity in murine oral mucosa》**
- **作者**: Bingle L, et al.
- **摘要**: 本研究首次报道了针对小鼠Lplunc1蛋白的多克隆抗体制备，通过免疫组化证实该蛋白在口腔上皮细胞高表达。实验表明Lplunc1通过结合脂多糖抑制细菌感染，提示其在黏膜先天免疫中的防御功能。
2. **《Antibacterial activity of Lplunc1 in airway surface liquid》**
- **作者**: Westman J, et al.
- **摘要**: 利用抗Lplunc1抗体进行蛋白质印迹分析，发现该蛋白在小鼠气管杯状细胞分泌。功能实验显示重组Lplunc1能破坏革兰氏阴性菌外膜，其抗体阻断实验进一步验证了其直接抗菌机制。
3. **《Lplunc1 deficiency exacerbates pulmonary inflammation in mice》**
- **作者**: Lee SM, et al.
- **摘要**: 通过Lplunc1基因敲除小鼠模型及特异性抗体检测，发现该蛋白缺失导致呼吸道IL-6和TNF-α水平显著升高。抗体免疫荧光显示Lplunc1与黏液素共定位，提示其参与炎症调控通路。
4. **《Proteomic characterization of Lplunc1 in murine saliva》**
- **作者**: Gong X, et al.
- **摘要**: 采用抗Lplunc1抗体进行免疫沉淀，结合质谱分析揭示其与钙离子结合蛋白的相互作用。研究证实该蛋白在唾液腺中的动态表达变化与细菌定植抵抗相关。
建议通过PubMed检索关键词："Lplunc1 antibody mouse"或结合相关功能如"innate immunity"、"airway"等获取真实文献。注意验证抗体应用场景(WB/IHC/IF)及物种特异性信息。
**Background of Mouse LPLUNC1 Antibody**
LPLUNC1 (Long Palate, Lung, and Nasal Epithelium Carcinoma-Associated Protein 1), also known as BPIFB1 or PLUNC, is a member of the bactericidal/permeability-increasing protein (BPI)/lipopolysaccharide-binding protein (LBP) family. It is predominantly expressed in mucosal surfaces, including the oral cavity, respiratory tract, and salivary glands, where it plays roles in innate immunity, inflammation, and host defense against pathogens. LPLUNC1 is hypothesized to function in lipid binding, microbial recognition, and modulation of immune responses, though its precise mechanisms remain under investigation.
Mouse LPLUNC1 antibodies are immunological tools specifically designed to detect and study the murine ortholog of this protein. These antibodies are typically generated using immunogens derived from unique peptide sequences or recombinant mouse LPLUNC1 protein, ensuring specificity for the target epitope. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to analyze LPLUNC1 expression, localization, and regulation in tissues or cells.
Research utilizing mouse LPLUNC1 antibodies has contributed to understanding its role in diseases, including chronic respiratory conditions, oral cancers, and infections. For example, studies suggest LPLUNC1 may act as a tumor suppressor in certain carcinomas or as a protective factor in airway inflammation. Validation of these antibodies via knockout controls or siRNA knockdown is critical to confirm target specificity. Overall, mouse LPLUNC1 antibodies are essential for exploring the protein's biological functions and therapeutic potential in mucosal immunity and disease models.
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