| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LOH12CR3; 9130403P13Rik |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MANSC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PIK3R1抗体的3篇参考文献的简要信息(注：内容基于模拟文献概括，实际文献请通过学术数据库查询)：
1. **文献名称**：*PIK3R1 mutations in cancer: a review of molecular mechanisms and therapeutic targeting*
**作者**：Samuels, Y. et al.
**摘要**：本文综述了PIK3R1基因突变在多种癌症中的功能影响，通过使用特异性抗体进行Western blot和免疫组化分析，揭示了突变导致PI3K-AKT信号通路异常激活的机制，并提出靶向该通路的潜在治疗策略。
2. **文献名称**：*Dysregulation of PIK3R1 in endometrial carcinoma: correlation with clinical outcomes*
**作者**：Dillon, L.M. et al.
**摘要**：研究利用抗PIK3R1抗体对子宫内膜癌组织进行免疫组化检测，发现PIK3R1蛋白表达水平与肿瘤分期和患者生存率显著相关，提示其可作为预后标志物。
3. **文献名称**：*Functional characterization of PIK3R1 variants in insulin resistance using CRISPR models*
**作者**：Taniguchi, C.M. et al.
**摘要**：通过CRISPR技术构建PIK3R1突变细胞模型，结合抗体介导的蛋白检测，证实特定突变会干扰胰岛素受体信号传导，导致代谢紊乱，为糖尿病机制研究提供依据。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“PIK3R1 antibody”或“PIK3R1 signaling”。
The PIK3R1 antibody is a crucial tool for studying the phosphatidylinositol 3-kinase (PI3K) regulatory subunit 1. encoded by the *PIK3R1* gene. This protein, p85α, serves as a regulatory component of the class IA PI3K complex, which plays a central role in intracellular signal transduction pathways, including the PI3K/AKT/mTOR axis. These pathways regulate critical cellular processes such as growth, proliferation, survival, and metabolism. The p85α subunit stabilizes the catalytic p110 subunit and modulates PI3K activity in response to extracellular signals, including growth factors and insulin. Dysregulation of this pathway is implicated in cancers, immune disorders, and metabolic syndromes.
PIK3R1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). They also help investigate the functional impact of *PIK3R1* mutations, such as somatic alterations in tumors (e.g., breast, colorectal cancers) or germline mutations linked to rare disorders like SHORT syndrome and AGAMA (autosomal dominant aggressive metabolic syndrome). Certain mutations disrupt p85α's inhibitory role, leading to constitutive PI3K activation and oncogenic signaling.
Clinically, PIK3R1 antibodies aid in identifying pathway hyperactivity for diagnostic or prognostic purposes. They also support therapeutic development, as PI3K inhibitors are being explored for cancer and metabolic disease treatment. However, challenges remain in distinguishing isoform-specific functions and avoiding cross-reactivity with related regulatory subunits (e.g., p85β, p55γ). Overall, these antibodies are vital for unraveling the molecular basis of PI3K-related diseases and advancing targeted therapies.
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