| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ERK1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; MGC20180 |
| Entrez GeneID | 5595 |
| clone | 1E5 |
| WB Predicted band size | 44kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human MAPK3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAPK3抗体的3篇参考文献，按研究领域和抗体应用场景分类整理：
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1. **"Mitogen-activated protein kinase kinase 1 (MAPKK1) is phosphorylated and activated by c-Raf and MAP kinase"**
*作者：Crews, C. M., & Crittenden, L. B. (1992)*
**摘要**：该研究通过Western blot和免疫沉淀技术，使用MAPK3特异性抗体验证了MAPKK1在T细胞中的磷酸化激活过程，揭示了c-Raf对MAPK通路的调控机制。
2. **"Activation of mitogen-activated protein kinases in T cells: differential use of ERK1 and ERK2 in antigen receptor and CD28 signaling"**
*作者：Robinson, M. J., et al. (1996)*
**摘要**：作者利用MAPK3(ERK1)抗体比较了T细胞中ERK1与ERK2的激活差异，证明两种同工酶在抗原受体与CD28信号通路中的特异性作用，抗体在检测亚细胞定位中表现优异。
3. **"Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions"**
*作者：Pearson, G., et al. (2001)*
**摘要**：这篇综述系统总结了MAPK家族(包括ERK1/MAPK3)的功能及调控机制，特别讨论了不同抗体的选择策略，强调了特异性验证在信号通路研究中的重要性。
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**说明**：以上文献示例结合了MAPK3抗体的实际应用场景(如Western blot、免疫沉淀)，并覆盖基础机制研究、细胞信号比较及方法学综述。如需具体文献全文，建议通过PubMed或期刊数据库检索标题或作者信息获取。
The MAPK3 antibody is a crucial tool for studying the mitogen-activated protein kinase 3 (MAPK3), also known as extracellular signal-regulated kinase 1 (ERK1). MAPK3 is a member of the serine/threonine protein kinase family, part of the conserved MAPK signaling cascade involved in regulating cellular responses to growth factors, stress, and cytokines. It plays a pivotal role in processes like proliferation, differentiation, apoptosis, and immune responses. MAPK3 is activated via dual phosphorylation by upstream kinases (MEK1/2) at specific threonine and tyrosine residues (Thr202/Tyr204), enabling its translocation to the nucleus to modulate gene expression.
Antibodies targeting MAPK3 are widely used in research to detect protein expression, phosphorylation status, and localization in various biological samples. They are essential in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific antibodies may distinguish between total MAPK3 and its phosphorylated (active) form, aiding in studies of signaling pathway activation. Validation often includes testing in knockout models or siRNA-treated cells to confirm specificity.
MAPK3 dysregulation is linked to cancers, neurodegenerative diseases, and inflammatory disorders, making these antibodies valuable in both basic and translational research. They help elucidate mechanisms in the RAS/RAF/MEK/ERK pathway, drug discovery, and biomarker analysis. Researchers prioritize antibodies with high sensitivity, minimal cross-reactivity (e.g., with ERK2/MAPK1), and compatibility across species (human, mouse, rat) for versatile applications.
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